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The proteins were incubated with 0.67 mg/ml DTNB and 10 U/ml glutathione reductase in 0.1 M potassium phosphate buffer with 5 mM EDTA disodium salt, pH 7.5

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Finally, glutathione deficiency is known to contribute to oxidative stress, and has an important role in aging, as well as in the pathogenesis of different diseases, such as neurodegenerative diseases, liver and kidney disorders, cystic fibrosis, diabetes, and cardiovascular illness