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Lactate dehydrogenase (LDH) Hemolysate was treated with the LDH reagent [a mixture of reagent 1 (80 mM Tris, 1.6 mM pyruvate, and 200 mM NaCl) and reagent 2 (0.2 mM NADH) at a ratio of 4:1], and incubated at 37˚C

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