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Study endpoints are not interchangeable: Total testosterone, which includes hormone bound to carrier proteins Free testosterone, the unbound fraction LH and FSH, which show the pituitary responding, one step before the final output SHBG, which changes how much testosterone is available to tissues Estradiol, which reflects conversion downstream A rise in LH shows the signal arriving at the pituitary, and whether testosterone followed is a separate measurement
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10.1016/S0014-5793(01)02519-4 FEBS Lett 94 MizushimaN