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The outcome of this competition depends from the Cu(II)-binding affinities and concentrations of HSA, Cu•HSA and His according to the following simplified reaction scheme: HSA and His are present in the blood serum at average 650 μM and 75 μM concentration respectively 22,42,43,44

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Glutathione-S-transferases (GSTs) primarily work on GSH conjugation, suggesting their involvement in GSL metabolism